Adeno-Associated Viral Vector Service

Adeno-Associated Viral Vector Service

Baseceuticals is the premier global biotech company which has confidence to provide adeno-associated viral vector service for gene delivery in an efficient way. We have a team of passionate and talented scientists who have a wealth of knowledge and extensive experience in gene delivery vectors. In addition, relying on our proprietary technologies and mature platform, we offer comprehensive adeno-associated viral vector service for global customers to meet customers' needs in basic research and preclinical application. Baseceuticals is your reliable partner and work closely with you to accelerate the project progress.

Adeno-associated viruses (AAVs) have become essential therapeutic gene delivery vectors. Adeno associated virus belongs to the parvoviridae family with an icosahedral symmetry morphology, and comprises a ~ 5 kb single-stranded deoxyribonucleic acid (DNA) genome with two inverted terminal repeats (ITRs) on both ends and flanked by two open reading frames (ORFs). One is structural rep gene indicating AAV life cycle required proteins and the other is packaging cap gene for capsid proteins.

Schematic representation of the basic components of a gene insert packaged inside recombinant AAV gene transfer vector Fig. 1 Schematic representation of the basic components of a gene insert packaged inside recombinant AAV gene transfer vector

The AAVs are popular gene delivery vectors because they are able to package and express foreign genes in a broad range of tissues. AAV vectors are non-pathogenic, eliciting little immune response and having potentials for long-lasting transgene expression in both quiescent and dividing cells, therefore, they are recognized as one of the major delivery vectors for research and gene therapy applications. In addition, recombinant AAVs can be manufactured and purified at a high titer, making them available for clinical use. There are gene therapy commercial products in the market such as Glybera® with AAV1, Luxturna® with AAV2 and Zolgensma® with AAV9.

Cell entry and trafficking of the adeno-associated virus (AAV) vector Fig. 2 Cell entry and trafficking of the adeno-associated virus (AAV) vector

Features of Adeno-Associated Viral Vectors for Gene Delivery

  • Little immunogenicity
  • Tissue-specific targeting
  • Stable and long-lasting expression for foreign genes
  • Safety

Adeno-Associated Viral Vector Service Provided by Baseceuticals:

  • Adeno-Associated Viral Vector design and construction

Highly efficient tailor-made AAV vector design and construction services with justifiable cost are provided by Baseceuticals' highly experienced teams. Different adeno-associated viral vectors are available for shRNA, human ORF cloning according to the different needs of customers. High GC content, large and highly repetitive sequences can be addressed in our excellent platform.

  • Selection of viral capsid serotype

Baseceuticals furnishes AAV capsid serotype selection services to ensure optimal gene delivery with a list below of our suggested AAV serotypes for different tissue types. If you are unsure about which AAV serotype could best fit your project, please contact our experts for advice on your selection.

Tissue typeSuggested AAV Capsid serotypes
Central Nervous SystemAAV1, AAV5, AAV8, AAV9, AAV-DJ
HeartAAV1, AAV8, AAV9
KidneyAAV2, AAV9
LiverAAV8, AAV9, AAV-DJ
LungAAV5, AAV6, AAV9
MuscleAAV1, AAV6, AAV8, AAV9
PancreasAAV8
RetinaAAV1, AAV2, AAV5, AAV8
  • Recombinant AAV vector packaging

Baseceuticals provide recombinant AAV vector packaging services based on the plasmid-mediated delivery into packing cell lines. After gradient ultracentrifugation, purification and RT-qPCR-determined titering, recombinant AAV vector particles are ready-to-use for in vitro or in vivo gene therapy applications.

Workflow

Adeno-Associated Viral Vector Service

Advantages of Adeno-Associated Viral Vector Service in Baseceuticals

  • Quick response with free consultation
  • Professional research team with years of experience
  • High efficiency and high quality service with satisfaction guaranteed
  • Fast turnaround time to strictly follow the timeline
  • Customized services according to the specific needs
  • Cost-effective price

Baseceuticals provides comprehensive adeno-associated viral vector services from vector design and construction to GMP production for clinical applications. Our talent experts will propose the best strategy to customers depending on the advanced technologies and years of experience. Based on the commitment of prompt communication and on-time reporting, our researchers will ensure the high-efficiency services to meet the strict project timelines. If you have any additional requirements or questions, please do not hesitate to contact us at or 1-631-626-9181. Our expert team is pleased to assist you in every way we can.

References

  1. Naso, M. F., Tomkowicz, B., Perry, W. L., & Strohl, W. R. (2017). Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs, 31(4), 317–334.
  2. Martini, S. V. , Rocco, P. R. M. , & Morales, M. M. (2011). Adeno-associated virus for cystic fibrosis gene therapy. Brazilian Journal of Medical and Biological Research, 44(11), 1097-1104.

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