CRISPR/Cas9 Service

CRISPR/Cas9 Service

As a leading provider of gene editing service, Baseceuticals is proud to offer comprehensive CRISPR/Cas9 services. We have developed an excellent platform for gene editing with high quality and high efficiency. Depending on the excellent platform and extensive experience, our staffs have succeeded in numerous CRISPR/Cas9 projects, including gene knockout, site-directed mutation, gene knock-in and precise base editing. At Baseceuticals, one-stop CRISPR/Cas9 services are available from sgRNA design/construction to cell line generation/verification and animal model generation. Combing with preclinical animal model production, functional analysis of diseases and target therapies, our scientists will work with you closely to assist you in accelerating the project progress.

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) is a technology to edit genome through removing, adding or altering parts of the DNA sequence. The CRISPR/Cas9 system is composed of two parts, namely guide RNA (gRNA) and Cas9. Guide RNA recruits Cas9 endonuclease to a specific region of the genomic DNA, resulting in a double strand break. Researchers make use of this technology to introduce changes in the genome. The CRISPR/Cas9 system greatly simplifies genome editing and can achieve targeted gene correction, which provides the potential for gene therapy applications.

CRISPR/Cas9-based gene modification Fig. 1 CRISPR/Cas9-based gene modification

CRISPR/Cas9 Service Provided by Baseceuticals:

  • SgRNA design and vector construction service

Our scientists will optimize the guide RNA sequences for maximum efficiency and minimum off-target effects. In addition, we offer target sequence cloning service into suitable vectors based on the specific need.

  • Cell line generation service

Based on highly optimized and efficient CRISPR/Cas9 gene editing strategies and protocols, we engineer a variety of cell lines including cancer, blood lineage and stem cell lines.

  • Genetically modified animal model generation service

We utilize CRISPR/Cas9 technology to rapidly and efficiently generate different animal models for customers. Various species are available, including mouse, rat, zebrafish and C.elegans.

  • Off-target prediction and analysis service

We employ different assays to analyze CRISPR off-target effects, including PCR-based assay, whole genome sequencing, GUIDE-seq and CIRCLE-seq.

Advantages of CRISPR/Cas9 Service in Baseceuticals

  • Complete solution: Comprehensive services include tool design and construction, functional validation, donor design and construction, cell line and animal model development.
  • Flexible donor design: Knock-in, knockout, mutagenesis, tagging, promoter swapping are available according to your requirements.
  • Validation available: DNA level or protein level validations are available.
  • Sequence guaranteed: all constructs are sequence verified and guaranteed.
  • High efficiency and high specificity
  • Competitive price

Baseceuticals has years of experience in gene editing by using CRISPR/Cas9 technology. We provide efficient and reliable results at the fast turnaround time and the competitive price. We are specializing in building efficient systems and procedures to meet the various needs and timelines of clients working in the CRISPR/Cas9. There is no doubt Baseceuticals will be the best partner during your research. For further information about our CRISPR/Cas9 service, please do not hesitate to contact us at or 1-631-626-9181.

Reference

  1. Tian, X., Gu, T., Patel, S., Bode, A. M., Lee, M.-H., & Dong, Z. (2019). CRISPR/Cas9 – An evolving biological tool kit for cancer biology and oncology. Npj Precision Oncology, 3(1).

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