TALEN Service

TALEN Service

As a leading provider of gene editing service, Baseceuticals has confidence to provide TALEN service to the global customers. With talented staff, advanced technologies and state-of-the-art facilities, Baseceuticals offers complete custom services for TALEN-based targeted genomic modification, including TALEN design and construction, donor design and construction, functional validations, stable cell line establishment, and transgenic animal models generation. At Baseceuticals, we take the time to analyze every project and propose the best strategy to ensure the various demands. Our dedicated scientists and technical support specialists are ready to partner with you to use TALEN technology for genome editing, accelerating your research program.

A transcription activator-like effector nuclease (TALEN) is a widely applicable technology for targeted genome editing. It is an artificial nuclease that contains customizable DNA-binding domain and a FokI nuclease domain. The mechanism of genome editing through TALEN is by inducing double-strand breaks (DSB). This repair mechanism causes mistakes through insertion, deletion, chromosomal rearrangement. And any mistake could lead to the gene products coded at the point non-functional. Alternatively, DNA could be introduced into the genome by NHEJ in the existence of foreign double-stranded DNA fragments. TALEN has a wide range of applications in gene therapy. For example, it is used to engineer stably modified embryonic stem cell and induced pluripotent stem cell clones. In addition, it is a promising method to correct the genetic errors that underlie disease such as sickle cell disease.

Schematic illustration of the TALEN system. (A) Binding of the customized TALENs on the genome. (B) Domain structure of homodimeric and heterodimeric TALENs. Fig. 1 Schematic illustration of the TALEN system. (A) Binding of the customized TALENs on the genome. (B) Domain structure of homodimeric and heterodimeric TALENs.

TALEN Service Provided by Baseceuticals

  • Donor design and construction service

Customized plasmids are designed to specifically transfer your gene of interest. Our vectors can combine with the selection markers or other genetic elements according to the specific need.

  • Functional validation service

We run a T7E1 cleavage assay to detect the presence of the indels created by TALEN-mediated NHEJ repairs at the specific target site of the chromosome.

  • Stable cell lines service

We offer single colony of stable cell line with TALEN-mediated genome modifications, and create cell banks of monoclonal stable cell lines.

  • Transgenic animal models service

We offer transgenic animals with TALEN-mediated genome modifications for our clients. We have established numerous animal models of human disease, which are widely used for gene research.

Our Advantages of TALEN Service

  • One-stop service to meet
  • Target any sequence in any cells
  • Highly sequence-specific gene editing
  • Flexible TAL effector design of binding and functional domains
  • Fastest delivery of TAL effector plasmids or mRNA transcripts
  • Excellent platform, advanced equipment and technologies
  • Reliable results provided in the shortest period
  • Competitive price

With years of experience in genome editing field, Baseceuticals provides the most excellent TALEN service for gene editing. Our talent experts are dedicated to use TALEN technology to edit genome with high efficiency and high quality. We provide one-stop TALEN service for gene editing including designing, synthesizing, validating your TALEN, stable cell line and transgenic animal models generation to meet your various needs. We make sure every step is well arranged and strictly meet the timeline. For further information about our TALEN service, please feel free to contact us at or 1-631-626-9181.

Reference

  1. Naitou, A., Kato, Y., Nakanishi, T., Matsuura, T., & Watanabe, H. (2015). Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna. Biology Open, 4(3), 364–369.

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